Jean X. Jiang, Ph.D.

• Professional Background

  Education

  • 1995 - Postdoctoral Fellowship - Cell Biology - Harvard Medical School,
  • 1991 - PhD - Biochemistry - State University of New York at Stony Brook
  • 1986 - BS - Biochemistry - Nanjing University

  Training

  • 2012 - Leadership (LEAD) Academy - University of Texas System

  Highlights

  National Research Service Award, NIH, San Antonio’s 40 under 40 Rising Star, University of Texas LEAD (Leadership) Fellow, CTRC Discovery of the Year Award, Ashbel Smith Professorship, Master Research Award for Distinguished Researcher, Presidential Distinguished Senior Research Scholar Award, Women in Medicine Month Honoree, Zachry Distinguished University Chair,  Fellow, American Society of Bone and Mineral Resear, Fellow, American Association for Advancement of Science (AAAS)

  Appointments

  • 1/2016-present - Associate Director, Joint Biomedical Engineering Program - UTHSCSA and UTSA
  • 1/2015-1/2016 - Interim Associate Director, Joint Biomedical Engineering Program - UTHSCSA and UTSA
  • 1/2015-present - COGS Chair, Joint Biomedical Engineering Program - UTHSCSA/UTSA
  • 2016-present - Member, Department Executive Committee (DEC), Dept of Biochemistry and Structural Biology, - UTHSCSA
  • 2016-2010 - Co-leader, Membrane Biology and Cell Signaling Graduate Track - UTHSCSA

• Instruction & Training

  • 1998- Present, Pre-Doctoral Student Supervision, UTHSCSA
  • 1998-Present, Membership on Supervising Committee, UTSA/UTHSCSA
  • 1997 - Present, Post-Doctoral Student Supervision, UTSA
  • 2000-present, Didactic lectures for Graduate Students, UTSA/UTHSCSA
  • 2003-present, Didactic lectures for Medical Students, UTHSCSA
  • 1998 - Present, Ph.D. Dissertations Directed, UTHSCSA
  • 1995, Teaching for Medical Students, Harvard Medical School

• Research & Grants

  Our current research interests are:

  1). To determine the gap junction or hemichannel-dependent and independent mechanisms of connexins in cell growth, differentiation and lens development. 

  2). To investigate the functional significance of gap junctions, hemichannels and integrins in signaling transmission, skeletal tissue remodeling and cancer metastasis of breast cancer and osteosarcoma. 

  Research profile

  Grants

  RO1 (AR076190)                    Jiang (MPI) and Wang (Contact PI)                           07/01/2019-06/30/2024

  NIH/NIAMS

  “Proteoglycans and age-related deterioration of bone toughness”

  The major objective of this study is to elucidate the underlying mechanism of proteoglycans in toughening of bone and its contribution to the age-related bone fragility.

   

  RO1 (AR072020)                    Jiang (PI)                                                                    08/01/2018-07/31/2023

  NIH/NIAMS

  “Connexin channels in transducing mechanical signals in bone”

  The major objective of this grant is to determine the specific mechanistic role of Cx43 hemichannels in mediating the anabolic effect of mechanical loading on the skeletal tissues.

   

  RO1 (EY012085)                    Jiang (PI)                                                                    03/01/2018-2/28/2023.

  NIH/NEI                                 

   “Intracellular Communication in the Eye lens”

  The major objective of this grant is to understand the roles of connexin hemichannels in mediating transport of glucose and antioxidiant in maintaining lens transparency and homeostasis, and cell protective function against oxidative stress.

  Role: PI

   

  BC161273                               Jiang (Lead PI) and An (Partner PI)                           09/30/2017-09/29/2021

  DoD

  “Development of hemichannel-targeting antibody therapies for breast cancer bone metastasis”

  The major objective of this grant is to develop a therapeutic antibody that targets connexin hemichannel and can be used to treat breast cancer bone metastasis.

   

  Research funding                   Jiang (PI)

  AlaMab Therapeutics                                                                                                  12/01/2017-11/30/2020

  “Development of therapeutics for disease Indications”

  The major objective of this grant funding is to develop antibody therapeutics and determine efficacies in several disease indications.

  Role: PI

   

  RO1 (CA196214)                    Jiang (Contact PI) and Sun (MPI)                              02/01/2016-01/31/2021

  NIH/NCI

  “Connexin hemichannels in suppression of breast cancer bone metastasis”

  The major objective of this grant is to explore the mechanistic roles of activated Cx43 hemichannels in osteocytes in inhibition of breast cancer cell migration and bone metastasis and the involvement of purinergic signaling in cancer cells.

  Role: Contact PI.                                                                                           

   

  Research Grant (AQ-1507)                Jiang (PI)                                                        06/01/2019-05/31/2022           

  Welch Foundation

  “Modulating hemichannel activities using targeting antibodies”

  The objective of this project is to use structural biology, antibody and microscopic approaches to understand the gating mechanism of connexin hemichannels.

   

  Research grant                       Jiang (PI)                                                                    07/01/2019-01/31/2020

  President’s Translational and Entrepreneurial Research Fund/UTHSCSA 

  “Novel small molecule, chemical compounds in the treatment of cancer metastasis”

  The objective of this project is to test the efficacy of novel chemical compounds on suppression of cancer bone metastasis.

  Role: PI

   

  Zachry Distinguished University Chair Endowment   Jiang (PI)                                03/20/2019-

  UTHSCSA

  University endowment for the holder of Zachry Distinguished University Chair in Cancer Research    

• Service

  Institutional

  Search Committee for Chair and faculty of Dept BSB and othe Dept; Chair and member, PTEC; Reviewer, UTHSA internal grants; Chair and Member, Dept and Institutional PTEC; Chair and Member, Dept and Univ. XYZ compensation committee, Chair, Graduate Faculty Assembly; Chair, Biosafety Committee and Library Committee 

   

   

   

   

   

   

   

  National

  Chair, Eduction Coimmittee and Member, Executive Committee of ICMRS;  Mentor, ARVO Leadership Development Program; Mentor, ASBMR-National Research Network Mentoring Program; Faculty Advisor, BMEGS-SA; Mentor, Global Mentoring Program, ARVO; Member, Scientific Program Development Committee, IGJC; 

   

• Publications

  Jiang, J.X., and London, E. (1990). Involvement of denaturation-like changes in Pseudomonas exotoxin A hydrophobicity and membrane penetration determined by characterization of pH and thermal transitions.  J. Biol. Chem.  265, 8636-8641.

  Jiang, J.X., Abrams, F., and London, E. (1991). Folding changes in membrane-inserted diphtheria toxin that may play important roles in its translocation.  Biochemistry 30, 3857-3864.

  Jiang, J.X., Chung, L.A., and London, E. (1991). Self-translocation of diphtheria toxin across model membranes.  J. Biol. Chem. 266, 24003-24010.

  London, E., Ulbrandt, N.D., Tortorella, D., Jiang, J.X., and Abrams, F.S. (1993). Insight into membrane protein folding and translocation from the behavior of bacterial toxins: models for membrane translocation.  Society of General Physiologists Series. 48, 45-61.

  Jiang, J.X., Paul, D.L., and Goodenough, D.A. (1993). Posttranslational phosphorylation of lens fiber connexin 46: a slow occurrence.  Invest. Opthalmol. & Visual Sci. 34, 3558-3565.

  Jiang, J.X., White, T.W., Goodenough, D.A., and Paul, D.L. (1994). Molecular cloning and functional characterization of chick lens fiber connexin 45.6.  Mole. Biol.  Cell 5, 363-373.

  Jiang, J.X., White, T.W., Paul, D.L., and Goodenough, D.A. (1994). Molecular and functional characterization of lens fiber connexins.  Progress in cell research. ed. Y. Kanno et al, Elsevier Science Press. 4, 377-381

  Jiang, J.X., White, T.W., and Goodenough, D.A. (1995). Changes in connexin expression and distribution during chick lens development.  Dev. Biol. 168, 649-661.

  Jiang, J.X., and Goodenough, D.A. (1996). Heteromeric connexon formation in lens gap junctions.  Proc. Natl. Acad. Sci. USA . 93, 1287-1291.

  Jiang, J.X. and Goodenough, D.A. (1998). Retroviral expression of connexins in embryonic chick lens.  Invest. Opthalmol. Visual Sci. 39, 537-543. 

  Jiang, J.X. and Goodenough, D.A. (1998). Phosphorylation of lens-fiber connexins in lens organ cultures.  Eur. J. Biochem.  255, 37-44. PMID: 9692898

  Jiang, J.X. and Goodenough, D.A. (1998).  Expression of retroviruses containing connexins in chick embryonic lens in situ. Gap Junctions. ed. R. Werner,  IOS Press. 96-101. 10. 

  He, D., Jiang, J.X., Taffet, S. M. and Burt, J.M. (1999). Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells.  Proc. Natl. Acad. Sci. USA. 96, 6495-6500.

  Yin, X., Jedrzejewski, P., and Jiang, J.X. (2000).  Phosphorylation of lens fiber connexin 45.6 on Ser363 by casein kinase II and its functional significance. J. Biol. Chem. 275, 6850-6856. PMID: 10702244

  Gu, S., Roderick, H.L., Camacho, P., and Jiang, J.X. (2000). Identification and characterization of an amino acid transporter expressed differentially in liver. Proc. Natl. Acad. Sci. 97, 3230-3235. PMID: 10716701

  Jiang, J.X. (2000).  Use of retrovirus to express connexins.  Methods in Molecular Biology, Gap Junction Protocols, ed. R. Bruzzone and C. Giaume, Humana Press Inc. pp 159-174. PMID: 11218647  

  Cheng, B., Zhao, S., Luo, J., Sprague, E., Bonewald, L.F. and Jiang, J.X. (2001).  Expression of functional gap junctions and regulation by fluid flow in osteocyte-like MLO-Y4 cells.  J. Bone Miner. Res. 16, 249-259. PMID: 11204425

  Gu, S., Roderick, H.L., Camacho, P., and Jiang, J.X. (2001).  Identification and characterization of an N-system amino acid transporter expressed in retina and its involvement in glutamine transport. J. Biol. Chem. 276, 24137-24144. PMID: 11325958

  Gu, S., Adan-Rice, D., Leach, R.J. and Jiang, J.X.  (2001).  A novel human amino acid transporter, PAAT1: cDNA cloning, chromosomal mapping, genomic structure, expression and functional characterization.  Genomics 74, 262-272. PMID: 11414754

  Cheng, B., Kato, Y., Zhao, S., Luo, J., Sprague, E., Bonewald, L.F., and Jiang, J.X. (2001).  Prostaglandin E2 is essential for gap junction-mediated intercellular communication between osteocytes in response to mechanical strain.  Endocrinology 142, 3464-3473. PMID: 11459792

  Yin, X., Gu, S., and Jiang, J.X. (2001).  The development-associated cleavage of lens connexin 45.6 by caspase-3-like protease is regulated by casein kinase II-mediated phosphorylation. J. Biol. Chem. 276, 34567-34572. PMID: 11448971

  Jiang, J.X., and Cheng, B. (2001). Mechanical stimulation of gap junctions in bone osteocytes is mediated by prostaglandin E2. Cell Communica. Adhesion 8, 283-288. PMID: 12064603

  Yin, X., Gu, S., and Jiang, J.X. (2001).  Regulation of lens connexin 45.6 by apoptotic protease, caspase-3.  Cell Communica. Adhesion 8, 355-359. PMID: 12064621

  Choi, D., Oh, H.J., Chang, U.J., Koo, S.K., Jiang, J. X., Hwang, S.Y., Lee, J.D., Yeoh, G.C., Shin, H.S., Lee, J-S., and Oh, B.  (2002).  In vivo differentiation of mouse embryonic stem cells into hepatocytes.  Cell Transplant 11, 359-368.

  Jiang, J.X. Yin, X., Gu, S., Yu, X.S., Siller-Jackson, A., Priyadarshini, P., and Villegas, C. (2002).  Function and regulation of gap junctions and connexins in the vertebrate eye lens.  Recent Research Developments in Biological Chemistry, ed. S.G. Pandalai, Research Signpost Press Inc. 307-318.

  Gu, S., Yu, X. S., Yin, X., and Jiang, J.X. (2003).  Stimulation of lens cell differentiation by a gap junction protein Cx45.6.  Invest. Opthamol. Vis. Sci. 44, 2103-2111. PMID: 12714649

  Gu, S., Langlais, P., Liu, F., and Jiang, J.X. (2003).  mNAT3, an N-system amino acid transporter expressed in hepatocytes and regulated by an insulin-activated and PI3 kinase-dependent signaling.  Biochem. J. 371, 721-731. PMID: 12537539  

  Jiang, J.X., and Cherian, P.P. (2003). Hemichannels formed by Cx43 play an important role in the release of prostaglandin E2 by osteocytes in response to mechanical strain. Cell Communica. Adhesion 10, 259-264. PMID: 14681026

  Cherian, P.P., Cheng, B., Gu, S., Sprague, E., Bonewald, L.F., and Jiang, J.X. (2003). Effects of mechanical strain on the function of gap junctions in osteocytes is mediated through the prostaglandin EP2 receptor.  J. Biol. Chem. 278, 43146-56. PMID: 12939279

  Gu, S., and Jiang, J.X. (2003).  A novel gene family-solute carrier family (Slc38a) representing system N and A-related amino acid transporters-A review.  J. Lanzhou Univ. 39, 28-34.

  Yu, X.S., and Jiang, J.X. (2004). Interaction of major intrinsic protein (aquaporin-0) with fiber connexins in lens development.  J. Cell Sci. 117, 871-880. PMID: 14762116

  Yu, X.S., Yin, X., Lafer, E.M. and Jiang, J.X. (2005). Developmental regulation of the direct interaction between the intracellular loop of connexin 45.6 and the C-terminus of major intrinsic protein (aquaporin-0).  J. Biol. Chem. 280, 22081-22090. PMID: 15802270

  Cherian, P.P., Siller-Jackson, A, J., Gu, S., Wang, X., Bonewald, L.F., Sprague, E. and Jiang, J.X. (2005).  Hemichannels formed by connexin 43 provide a novel pathway for the release of prostaglandin E2 by osteocytes in response to mechanical strain. Mol. Biol. Cell 16, 3100-3106.  (*Highlighted as a hottest paper, Science STKE 291, tw242, 2005). PMCID: 1165395

  Gu, S., Villegas, C., and Jiang, J.X. (2005).  Differential regulation of amino acid transporter SNAT3 by insulin in hepatocytes.  J. Biol. Chem. 280, 26055-26062. PMID: 15899884Jiang, J.X. and Gu, S. (2005).  Gap junction-independent actions of connexins.  Biochem. Biophys. Acta. 1711, 208-214. PMCID: PMC1831832

  Cherian, P.P., Siller-Jackson, A, J., Gu, S., Wang, X., Bonewald, L.F., Sprague, E. and Jiang, J.X. (2005).  Mechanical strain opens connexin hemichannels in osteocytes: a novel mechanism for the release of prostaglandin. Sci. Signal. 16, 3100.

  Gluhak-Heinrich, J., Gu, S., Pavlin, D., and Jiang, J.X. (2006).  Mechanical loading stimulates expression of connexin 43 in alveolar bone cells in the tooth movement. Cell Communica. Adhsion. 13, 115-125 PMID: 16613785

  Jiang, J.X., Siller-Jackson, A.J., and Burra, S. (2007).  Roles of gap junctions and hemichannels in bone cell functions and in signal transmission of mechanical stress. Front. Biosci. 12, 1450-1462 PMCID: PMC1797155

  Moret C., Dave, M.H., Schulz, N., Jiang, J.X., Verrey, F., Wagner, C.A.  (2007). Regulation of renal amino acid transporters during metabolic acidosis.  Am. J. Physiol. Renal Physiol. 292, F555-566 

  Banks E.A., Yu, X.S, Qian, S. and Jiang, J.X. (2007). Promotion of lens epithelial-fiber differentiation by C-terminus of connexin 45.6, a role independent of gap junction communication J Cell Sci. 12, 3602-3612. (*highlighted “in this issue”).  PMID: 17895360

  Cherian, P.P., Xia, X., and Jiang, J.X. (2008) Role of gap junction, hemichannels, and connexin 43 in mineralizing MLO-A5 cells in response to intermittent and continuous application of parathyroid hormone. Cell Communica. Adhsion. 15, 43-54. PMID: 18649177

  Yin, X., Liu, J. and Jiang, J.X. (2008) Lens fiber connexin turnover and caspase-3-mediated cleavage are regulated alternately by phosphorylation. Cell Communica. Adhsion. 15, 1-11. PMCID: 2719281

  Kihara, A.H., Paschon, V., Akamine, P.S., Saito, K.C., Leonelli, M., Jiang, J.X., Hamassaki, D.E. and Britto, L.R.G. (2008) Connexin expression during histogenesis of the chick retina. Dev. Neurobiol. 68, 1287-302.

  Siller-Jackson, A.J., Burra, S., Gu, S., Bonewald, L.F., Sprague, E., and Jiang, J.X. (2008) Adaptation of connexin 43-hemichannel prostaglandin release to mechanical loading J. Biol. Chem. 283, 26374-26382. (*highlighted in “Not to be missed”, BoneKEy, a knowledge environment for the bone field). PMCID: PMC2546557

  Banks, E.A., Toloue, M.M., Shi, Q., Zhou, Z.J., Liu, J., Nicholson, B.J. and Jiang, J.X. (2009) Connexin mutation that causes dominant congenital cataracts inhibits gap junctions, but not hemichannels, in a dominant negative manner. J. Cell Sci. 122: 378-388. PMID: 19126675

  Biswas, S.K., Jiang, J.X. and Lo, W-K. (2009). Gap junction remodeling associated with cholesterol redistribution during fiber cell maturation in the adult chicken lens. Mole. Vis. 15:1492-1508. PMID: 19657477

  Burra, S., and Jiang, J.X. (2009). Connexin 43 hemichannel opening associated with prostaglandin E2 release is adaptively regulated by mechanical stimulation. Communi. & Integra. Biol. 2(3). PMID: 19641742. PMCID:2717532

  Rath, A.L., Bonewald, L.F., Ling, L., Jiang, J.X., Van Dyke,, M.E., and Nicolella, D.P. (2009) Correlation of cell strain in single osteocytes with intracellular calcium, but not intracellular nitric oxide, in response to fluid flow. J. Biomech. 43, 1560-1564. PMCID: PMC2866825

  Xia, X., Batra, N., Shi, Q., Bonewald, L.F., Sprague, E., and Jiang, J.X. (2010) Prostaglandin promotion of osteocyte gap junction function through transcriptional regulation of connexin 43 by GSK-3-β-catenin signaling. Mol. Cell. Biol. 30, 206-219. PMCID: PMC2798309

  Jiang, J.X. (2010). Gap junctions/hemichannel-depedent and independent roles of connexins in cataractogenesis and lens development. Cur. Mole. Med. 10, 851-63. PMID: 21091421

  Shi, Q., Banks, E.A., Yu, X.S., Gu, S., Lauer, J., Fields, G.B., and Jiang, J.X. (2010) An amino acid residue V362 plays a critical role in maintaining the structure of Connexin 50 C-terminus and in lens epithelial-fiber differentiation. J. Biol. Chem. 285, 18415-18422. PMCID:2881767

  Kitase, Y., Barragan, L., Jiang, J.X., Johnson, M.L., Bonewald, B.F. (2010). Mechanical induction of PGE2 in osteocytes blocks glucocorticoid induced apoptosis through both the β-catenin and PKA pathways. J. Bone Miner. Res. 25, 2381-92. PMCID: 3179278

  Xia, X., Kar, R., Gluhak-Heinrich, J., Yao, W., Lane, N. Bonewald, L.F., Biswas, S.K., Lo, W-K., and Jiang, J.X. (2010). Glucocorticoid induced autophagy in osteocytes. J. Bone Miner. Res. 25, 2479-2488. PMCID:3179284

  Burra, S., Nicolella. D.P., Francis, W.L., Freitas, C.J., Mueschke, N.J., Poole, C., and Jiang, J.X. (2010) Dendritic process of osteocytes is a mechanotransducer that induces the opening of hemichannels. Proc. Natl. Acad. Sci. 107, 13648-13653. PMCID: PMC2922284

  Biswas, S.K., Lee, J.E., Brako, L., Jiang, J.X. and Lo, W-K (2010) p junctions are selectively associated with interlocking ball-and-sockets but not protrusions in the lens. Mole. Vis. 16:2328-2341. PMID: 21139982

  Liu, J., Xu, J., Gu, S., Nicholson, B.J., and Jiang, J.X. (2010) Aquaporin 0 enhances gap junction coupling via its cell adhesion function and interaction with connexin 50. J. Cell Sci. 124, 198-206. PMCID: PMC3010190

  Burra, S., Nicolella. D.P., and Jiang, JX. (2010) Dark horse in osteocyte biology: Glycocalyx around the dendrites is critical for osteocyte mechanosensing. Communi. & Integra. Biol. 4, 48-50. PMCID: PMC3073269

  Bao, B., Jiang, J.X., Yanase, T., Nishi, Y., Morgan, J.R. (2011) Connexon-mediated cell adhesion drives microtissue self-assembly. FASEB J. 25, 255-264. PMCID: PMC3005422

  Orellana, J.A., Shoji, K.F., Abudara, V., Ezan, P., Amigou, E., Jiang, J.X., Sáez, J.C. and Giaume, C. (2011). Amyloid β-induced neuronal death involves glial and neuronal hemichannels. J. Neurosci. 31, 4962-4977

  Fang, X., Huang, T., Zhu, Y., Jiang, J.X., Wang, P., Kitamura, M., and Yao. J. (2011) Gap junction hemichannel exaggerates cadmium-induced oxidative stress and cell injury. Antioxid. Redox Signal. 14:2427-2439. PMCID: PMC3096519

  Orellana, J.A., Froger, N., Ezan, P., Jiang, J.X., Bennett, M.V.L., Naus, C.C., Giaume, C. and Sáez, J.C. (2011) ATP and glutamate released via astroglial connexin43 hemichannels mediate neuronal death through  activation of pannexin 1 hemichannels. J. Neuro. Chem. 118:826-840.

  Liu, J., Ek Vitroin, J.F., Weintraub, S.T., Gu, S., Shi, Q., Burt, J.M., Burt, J.M., and Jiang, J.X. (2011). Phosphorylation of connexin 50 by protein kinase A enhances gap junction and hemichannel function. J. Biol. Chem. 286:16914-28. PMCID: PMC3089535

  Burra, S. and Jiang, J.X. (2011). Regulation of cellular function by connexin hemichannels. Int. J. Biochem. Mol. Biol. 2:119-128. PMCID:3180094

  Jia, J., Yao, W., Min Guan, Dai, W., Quarles, D., Kar, R., Bonewald, L., Jiang, J.X., and Lane, N.E. (2011) Glucocorticoid dose determines osteocyte cell fate FESEB J. 25, 3366-3376. PMCID:3177583

  Shi, Q., Padmanabhan Iyer, R., Villegas, C.J., Gu, S., and Jiang, J.X. (2011) Membrane topological structure of SNAT4 neutral amino acid transporter. J. Biol. Chem. 286, 38086-38094. PMCID:3207422

  Orellana, J.A., Sáez, P.J., Elizondo, R., Cortés-Campos, C., Shoji, K.F., Contreras-Duarte, S., Figueroa, V., Velarde, V., Jiang, J.X., Nualart, F., Sáez, J.C., and García, M.A. (2012). Glucose increases intracellular Ca2+ in tanycytes via ATP released through connexin 43 hemichannels. Glia 60, 53-68.

  Batra, N., Kar, R., and Jiang, J.X. (2012). Gap junctions and hemichannels in signal transmission, function and development of bone. Biochem. Biophys. Acta. 1818:1909-1918. PMCID3440861

  Batra, N., Burra, S., Siller-Jackson, A.J., Gu, S., Xia, X., Weber, G., DeSimone, D., Bonewald, L.F., Lafer, E.M., Sprague, E., Schwartz, M.A., and #Jiang, J.X. (2012) Mechanical stress activates integrin α5β1 induces opening of connexin 43 hemichannels. Proc. Nat. Acad. Sci. 109:3359-64. PMCID:3295295 (highlighted in “Editor’s Choice”, Science, 335, 1021 (2012)

  Rosario, F. J., Schumacher M. A., Jiang J.X., Kanai, Y., Powell, Theresa L, and Jansson, T. (2012) Chronic maternal infusion of full-length adiponectin in pregnant mice down-regulates placental amino acid transporter activity and expression and decreases fetal growth. J. Physiol. 590:1495-509.  

  Kar, R., Batra, N., Riquelme, M. A. and Jiang, J.X. (2012) Biological role of connexin intercellular channels and hemichannels. Acta. Biochem. Biophys. 524:2-15

  Wang, K., Gu, S., Yin, X., Weintraub, S.T., Hua, Z. and Jiang, J.X. (2012) Developmental truncations of connexin 50 by caspases adaptively regulate gap junctions/hemichannels and protect cells against UV radiation. J. Biol. Chem. 287, 15786-15797. PMCID: 3346145

  Klionsky, D.J., Abdalla, F.C.,….Jiang, J.X., etc (2012). Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 8, 1-100.

  Batra, N. and Jiang, J.X. (2012). “INTEGRINating” the connexin hemichannel function in bone osteocytes through the action of integrin α5. Communi. & Integra. Biol. 5, 1-3.

  Padmanabhan Iyer, R., Gu, S., Nicholson, B.J., and Jiang, J.X. (2012) Residue cysteine 232 is important for substrate transport of neutral amino acid transporter, SNAT4. Int. J. Biochem. Mol. Biol. 3, 374-383.

  Loiselle, A.E., Jiang, J.X., Donahue, H.J. (2013). Gap junction and hemichannel functions in osteocytes. Bone 54, 205-212.

  Padmanabhan, R., Gu, S. Xu, J., Nicholson, B.J. and Jiang, J.X. (2013) Identification of a disulfide bridge important for transport function of SNAT4 neutral amino acid transporter. PloS One e56792.

  Yao, W. Dai, W., Jiang, J.X. and Lane, N.E. (2013). Glucocorticoids and osteocyte autophagy. Bone 54, 279-284.

  Kar, R., Riquelme, M.A., Werner, S., and Jiang, J.X. (2013) Connexin 43 channels protect osteocytes against oxidative stress-induced cell death. J. Bone Miner. Res. 28, 1611-1621. PMCID3688648

  Riquelme, M.A., Kar, R., Gu, S., Jiang, J.X. (2013) Antibodies targeting extracellular domain of connexins for studies of hemichannels.  Neuropharmacology 75, 525-532.

  Pei, C., Xu, Y., Jiang J.X., Cu, L-J., Li, L., and Qin, L. (2013). Application of sustained delivery microsphere of cyclosporine A for preventing posterior capsular opacification in rabbits. Inter. J. Opthalmol. 6, 1-7.

  Sáez, P.J., Shoji, K.F., Retamal, R., Harcha, P.A., Ramirez, G., Jiang, J.X., Von Bernhardi, R.; and Saez, J.C. (2013) ATP is required and advances the cytokine-induced gap junction formation in microglia in vitro. Mediators of Inflammation 2013, 216402.

  Sáez, P.J., Orellana, J.A., Vega-Riveros, N., Figueroa, V.A., Hernández, D.E., Castro, J.F., Klein, A.D., Jiang, J.X., Zanlungo, S. and Sáez, J.C. (2013) Disruption in connexin-based communication is associated with intracellular Ca2+ signal alterations in astrocytes from Niemann-Pick type C mice PLOS ONE. 8, e71361.  

  Ren, Q., Riquelme, M.A., Xu, J., Yang, X., Nicholson, B.J., Gu, S., and Jiang, J.X. (2013) Cataract-causing mutation of human connexin 46 impairs gap junction, but increases hemichannel function and cell death. PLOS ONE 8, e74732.

  Riquelme, M.A. and Jiang, J.X. (2013) Elevated intracellular Ca2+ signals by oxidative stress activate connexin 43 hemichannels in osteocytes. Bone Research 4, 355-361.

  Batra, N., Riquelme, M.A., Burra, S., and Jiang, J.X. (2014) 14-3-3θ facilitates plasma membrane delivery and function of mechanosensitive connexin 43 hemichannels. J. Cell Sci. 127, 137-146. PMCID3874784

  Zhou, J.X. and Jiang, J.X. (2014) Gap junction and hemichannel independent actions of connexins on cell and tissue functions – an update. FEBs Letters 588, 1186-1192.

  Lo, W-K., Biswas, S., Brako, L., Shiels, A., Gu, S., and Jiang, J.X. (2014) AQP0 targets interlocking domains to control integrity and transparency of eye lens. Invest. Opthalmol. Vis. Sci. 55, 12021-1212.

  Batra, N., Riquelme, M.A., Burra, S., and Jiang, J.X. (2014) Direct regulation of osteocytic connexin 43 hemichannels through AKT kinase activated by mechanical stimulation. J. Biol. Chem. 289, 10582-10591. PMCID4036178

  Zhang, J., Zhang, H., Zhang, M., Qiiu, Z., Wu, Y., Callaway, D.A., Jiang, J.X., Jing, L., Yang, T., Wang, M. (2014). Connexin43 hemichannels mediae small molecule exchange between chondrocytes and matrix in biomechanically stimulated temporomandibular joint cartilage. Osteoarthritis Cartilage 22, 822-830.

  Atintas, M.M., Moriwaki, K., Wei, C., Moller, C.C., Flesche, J., Li, J., Yaddanapudi, S., Faridi, M.H., Godel, M., Huber, T., Preston, R.A., Jiang, J.X., Kerjaschki, D., Sever, S., and Reiser, J. (2014) Reduction of proteinuria through podocyte alkaliization. J. Biol. Chem. 289, 17454-17467.

  Biswas, S.K., Brako, L., Gu, S., Jiang, J.X. and Lo, W-K. Regional changes of aquaporin 0 and connexin 50 associated with cortical cataract formation in the Emory mouse (2014) Exp. Eye Res. 127C, 132-142.

  Xu, H., Duan, J., Ning, D., Li, J., Liu, R., Yang, R., Jiang, J.X. and Shang, P. (2014). The role of Wnt signaling in fracture healing. BMB Reports 47, 666-672.

  Zhou, J.Z., Riquelme, M.A., Ellies, L.G., Sun, L-Z., Jiang, J.X. (2015) Differential impact of adenosine nucleotides released by osteocytes on breast cancer growth and bone metastasis. Oncogene 34, 1831-1842.

  Xu, H., Gu, S., Riquelme, M.A., Burra, S., Callaway, D.A, Cheng, H., Guda, T., Schmitz, J., Fajardo, R., Werner, S.L., Zhao, H., Shang, P., Johnson, M.L., Bonewald, L.F., and Jiang, J.X. Connexin 43 channels are essential for normal bone structure and osteocyte viability. (2015) J. Bone Miner. Res. 30, 436-448. PMCID:PMC431576

  Callaway, D.A, and Jiang, JX. (2015) Reactive Oxygen species and oxidative stress in osteoclastogenesis, skeletal aging and bone diseases J. Bone Miner. Metabol. 33, 359-370.

  Callaway, D.A., Riquelme, M.A., Sharma, R., Lopez-Cruzan, M., Herman, B.A. and Jiang, J.X. (2015) Caspase-2 modulates osteoclastogenesis through down-regulating oxidative stress. Bone 76, 40-48.

  Jiang, J.X., Riquelme, M.A., and Zhou, J.Z. ATP, a double-edged sword in cancer. (2015) Oncoscience 2, 673-674.

  Riquelme, M.A., Burra, S., Kar, R., Lampe, P.D., Jiang, J.X. (2015) MAPK activated by prostaglandin E2 phosphorylates connexin 43 and closes osteocytic hemichannels in response to continuous flow shear stress. J. Biol. Chem. 290, 28321-28328.

  Shi, Q., Gu, S., Yu, X.S., White, T.A., Banks, E.A., and Jiang, J.X. (2015) Connexin controls cell cycle exit and cell differentiation by directly promoting cytosolic localization and degradation of E3 ligase Skp2. Dev. Cell. 35, 483-496.

  Jiang, J.X and Penuela, S. (2016). Connexin and pannexin channels in cancer. BMC Cell Biol. 17 Suppl. 1:12. (Top three cited papers).

  Shi, Q and Jiang, J.X. (2016). Connexin arrests cell cycle through cytosolic retention of an E3 ligase Mole. Cell. Oncol. 3, e1132119.

  Wang, X., Xu, H., Huang, Y., Gu, S., and Jiang, J.X.  (2016) Coupling effect of water and proteoglycans on the in situ toughness of bone. J. Bone Miner. Res. 31, 1026-1029.

  Zhou, J.Z., Riquelme, M.A., Gu, S., Kar, R., Gao, X., Sun, L and Jiang, J. X. (2016) Osteocytic connexin 43 hemichannels suppress breast cancer growth and bone metastasis. Oncogene 35, 5597-5607. (Commentary by Bultynck,, G “The anti-metastatic micro-environment of the bone: Importance of osteocyte Cx43 hemichannels”. Biochim. Biophys. Acta. 2016, 1866:121-127)

  Padmanabhan Iyer, R., Gu, S., and Jiang, J.X. (2016) N-Glycosylation Influences Transport, but not Cellular Trafficking, of a Neuronal Amino Acid Transporter SNAT1. Biochem. J. 473, 4227-4242

  Klionsky, D.J., Abdalla, F.C.,….Jiang, J.X., etc (2016). Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 12, 1-222.

  Xu, H., Liu, R., Ning, D., Zhang, J., Yang, R., Riquelme, M.A., Li, J., Jiang, J.X., and Shang, P. (2017) Biological responses of osteocytic connexin 43 hemichannels to simulated microgravity J. Orthopaed. Res. 35, 1195-1202

  Xu, H., Ning, D., Zhao, D, Chen, Y., Zhao, D., Gu, S., Jiang, J.X., Shang, P. (2017) Blockage of hemichannels alters gene expression in osteocytes in high magneto-gravitational environment. Front. Biosci. 22, 783-794.

  Hu, Z., Shi W., Riquelme, M., Shi, Q., Biswas, S., Lo, W-K., White, T.W., Lo, Gu, S., and Jiang, J.X. (2017) Connexin 50 functions as an adhesive molecule and promotes lens cell differentiation. Sci. Report 7, 5298

  Roy, S., Jiang, J.X., Li, A-F., and Kim, D. (2017) Connexin channels and its role in diabetic retinopathy. Prog. Ret. Eye Res. 61, 35-59

  Xu, H., Duan, Ren, L., Chen, M., J., Yang, R., Yang, R., Li, W., Zhao, D., Shang, P., and Jiang, J.X. (2017) Impact of flow shear stress on morphology of osteoblast-like IDG-SW3 cells. J. Bone Miner. Metab. 36, 529-536

  Tarzemany R., Jiang, G., Jiang, J.X., Larjava, H., and Hakkinen, L. (2017) Connexin 43 hemichannels regulate expression of wound healing-associated genes in human gingival fibroblasts. Sci. Report. 7, 14157.

  Iyyathurai, J., Wang, N., D'hondt, C., Jiang, J.X., Leybaert, L., and Bultynck, G. (2018) The SH3-binding domain of Cx43 participates in loop/tail interactions, critically controlling Cx43-hemichannel activity. Cell Mole. Life Sci. 293, 2573-2585.

  Wang X., Hua, R., Ahsan, A., Ni, Q., Huang, Y., Gu, S., and Jiang, J.X. (2018) Age-related deterioration of bone toughness is related to diminishing amount of matrix glycosaminoglycans (GAGs). J. Bone Miner. Res. Plus 2, 164-173

  Hu, Z., Riquelme, M.A., Wang, B., Brenner, R., Gu, S., and Jiang, J.X. (2018) Cataract-causing connexin 46 mutation alters its interaction with calmodulin and gating of hemichannels. J. Biol. Chem. 293, 2573-2585.

  Shi, W. Riquelme, M.A., Gu, S., Jiang, J.X. (2018) Connexin hemichannel mediates glutathione transport and protects lens fiber cells against oxidative stress. J. Cell Sci. 131, jcs212506

  Tarzemany, R., Jiang, G., Jiang, J.X., Gallant-Behm, C., Wiebe, C., Hart, D., Larjava, H., Häkkinen, L. (2018) Connexin 43 regulates the expression of wound healing-related genes in human gingival and skin fibroblasts. Exp. Cell Res. 367, 150-161.

  *Graham, S.V., *Jiang, J.X., and Mesnil, M. (2018) Connexins and pannexins: important players in tumorgenesis, metastasis and potential therapeutics.  International Journal of Molecular Sciences (*Co-first authors). 19, E1645

  Kar, R., Riquelme, M.A., Hua R., Jiang, J.X. (2018) Glucocorticoid-induced autophagy protects osteocytes against oxidative stress through activation of MAPK/ERK signaling. J. Bone Mineral Res. Plus 3, e10077.

  Werner, S.L., Sharma, R., Woodruff, K., Horn, D., Harris, S.E., Gorin, Y., Lee, D-Y., Hua, R., Gu, S., Fajardo, R.J., Habib, S.A. and Jiang, J,X. (2018) CSF-1 in osteocytes inhibits Nox4-mediated oxidative stress and promotes normal bone homeostasis. J. Bone Mineral Res. Plus 4, e10080

  Chen, Y., Chen, M., Xue, T., Li, G., Wang, D., Shang, P., Jiang, J.X., and Xu, H. (2019) Osteocytic connexin 43 channels affect fracture healing. J. Cell. Physiol. 234, 19824-19832

  Mondor, I., Baratin, M., Saro, L., Lagueyrie, M., Henri, S., Gentek, R., Suerinck, D., Jiang, J.X., and Bajénoff, M. (2019) Lymphatic endothelial cells are essential components of the subcapsular sinus macrophage niche. Immunity 50, 1453-1466.

  Ma, L., Hua, R., Tian, Y., Cheng, H. Fajardo, R.J., Pearson, J.J., Guda, T., Shropshire, D.B., Gu, S., and Jiang, J.X. (2019) Connexin 43 hemichannels protect bone loss during estrogen deficiency. Bone Res. 7, 1-12

  Li, G.; Zhang, L.; Wang, D.; AlQudsy, L.; Jiang, J.X.; Xu, H.; Shang, P. (2019) Muscle-bone crosstalk and potential therapies for sarco-osteoporosis. J. Cell. Biochem. 120, 14262-14273

  Gu, S., Biswas, S., Rodriguez,  L., Li, Z., Li, Y., Riquelme, M.A., Shi, W., Wang, K., White, T.W., Reilly, M., Lo, W-K., and Jiang, J.X. (2019) Connexin 50 and AQP0 are essential in maintaining organization and integrity of lens fibers. Invest. Opthamol. Vis. Sci. 60, 4021-4032.

  Riquelme, M.A., Cardenas, R.E., Xu, H., and Jiang, J.X. (2020) The role of connexin channels in the response of mechanical loading and unloading of Bone. Int. J. Med. Sci. 21, 1146

  Zhao, D., Liu, R., Li, G., Chen, M., Shang, P., Yang, H., Jiang, J.X. and Xu, H. (2020). Connexin 43 channels in Osteocytes Regulate Bone Responses to Mechanical Unloading. Front. Physiol. 11, 299.

  Badimon, A., Ayata, P., Strasburger, H.J., Chen, X., Nair, A., Ikegami, A., Graves, S.M., Sullivan, J.M., Ishikawa, M., Chan, A.T., Wang, Y-C., Loh, Y-H, E., Wheeler,M., Jiang, J.X., Sebra, R., Kenny, P.J., Surmeier, D.J., Quintana, F.J., Wake, H., Gradinaru, V., Schaefer, A. Negative feedback control of neuronal activity by microglia. Nature 1-7.

  Liu, J., Riquelme, M.A., Li, Z., Li, Y., Quan, Y., Pei, C., Gu, S., and Jiang, J.X. (2020) Intracellular delivery of glucose and glutathione in the lens by mechanosensitive hemichannels and integrins. J. Cell Biol. 219 (12).

  Riquelme, M.A. Cardenas, E.R. and Jiang, J.X. (2020) Osteocytes and bone metastasis Front. Endocrinol. 10, 2819.

  Chen, C-X., Luo, K-J, Yang, J-P., Huang, Y-C., Cardenas, E.R., Nicholson, B.J., and Jiang, J.X. (2020) Connexins and cAMP in cross-talk in cancer metastasis. Cancers 13, 58

  Hu, Z., Riquelme, M.A., Gu, S., and Jiang, J.X. (2020) Regulation of connexin 46 gap junctions and hemichannels by calcium and calcium binding protein calmodulin. Int. J. Med. Sci. 21, 8194

  Luo, K.J., Chen, C-X., Yang, J-P., Huang, Y-C., Cardenas, E.R., and Jiang, J.X. (2020) Connexins in lung cancer and brain metastasis. Front. Oncol. 10:599383

  Hua, R., Ni, Q., Eliason, T.D., Han, Y., Gu, S., Nicolella, D. P., Wang, X. and Jiang, J.X. (2020) Biglycan and chondroitin sulfate play pivotal roles in bone toughness via retaining bound water in bone mineral matrix. Matrix Biol.  94, 95-109

  Li, G; Zhang, L; Ning, K; Yang, B; Acosta, F.M; Shang, P; Jiang, J.X., Xu, H. (2021) Osteocytic connexin43 channels regulate bone-muscle crosstalk. Cells 10, 237.

  Zhang, C., Yan, Z., Maknojia, A., Riquelme, M.A., Gu, S., Booher, G., Wallace, D.J., Bartanusz, V., Goswami, A., Xiong, W., Zhang, N., Malder, M.J., An, Z., Sayre, N.L., and Jiang, J.X. (2021) Inhibition of astrocyte hemichannel improves recovery from spinal cord injury. JCI Insight 6, e134611.

  Pellefigues, C., Naidoo, K., Mehta, P., Schmidt, A., Jagot, F., Roussel, E., Yumnam, B., Chappell, S., Camberis, M., Jiang, J.X., Abboud Werner, S., Painter, C., Uluckan, O., Gasser, O., and Le Gros, G. (2021) Basophils promotes barrier dysfunctino and resolution in the atopic skin. J. Allergy Clin. Immunol. 148,799-812

  Zhang, J., Wu, Q., Johnson, C.B., Olsson, A., Slaughter, A., May, M., Weinhaus, B.,  D’Alessandro, A., Engel, J.D., Jiang, J.X., Koffron, J.M., Huang, L.F., Salomonis, N., Grimes, H.L. and Lucas, D. (2021) In situ mapping identifies distinct vascular niches for myelopoiesis. Nature 590, 457-462

  Chen, M., Zhang, L., Li, G., Jiang, J.X., Wang, D-E. and Xu, H. (2021) Primary osteocyte supernatants metabolomics profiling of two transgenic mice with connexin43 dominant negative mutants. Front. Endo 18;12:649994

  Riquelme, M.A., Gu, S., Hua, R. and Jiang, J.X. (2021) Mechanotransduction through coordinated actions of integrins, PI3K signaling and connexin hemichannels. Bone Res.9, 1-11

  Hua, R., Zhang, J., Riquelme, M.A., and Jiang, J.X. (2021) Connexin gap junctions and hemichannels link oxidative stress to skeletal physiology and pathology. Cur. Osteoporosis. Rep. 19, 66-74.

  Li, Z., Quan Y., Gu, S., Quan, Y., Varadaraj, K., and Jiang, J.X. (2021) Development of a potent in vivo model for studying congenital cataracts. Commun. Biol. 4,1-13

  Klionsky, D.J., Abdalla, F.C….Jiang, J.X., etc (2021). Guidelines for the use and interpretation of assays for monitoring autophagy.  Autophagy, 1-382

  Hua, R. and Jiang J.X. (2021) Small leucine-rich proteoglycans in physiological and biomechanical function of bone. Matrix Biology Plus 100063

  Tian, Y., Riquelme, M.A., Tu, C., Quan, Y., Liu, X., Sun, L-Z. and Jiang J.X. (2021) Osteocytic connexin hemichannels modulate oxidative bone microenvironment and breast cancer growth. Cancers 13, 6343

  Li, Y., Li, Z., Quan, Y., Cheng, H., Riquelme, M.A., Li, X-D., Gu, S. and Jiang, J.X. (2021) Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal and fibrosis. iScience 24, 102533.

  Liu, X., Riquelme, M.A., Tian, Y., Acosta, F.M., Gu, S. and Jiang, J.X. (2021) ATP inhibits breast cancer migration and bone metastasis through down-regulation of CXCR4 and purinergic receptor P2Y11. Cancers 13, 4293.

    Han, Y., Gomez, J., Hua, R., Xiao, P., Gao, W., Jiang, J.X., and Wang, X. (2021) Removal of glycosaminoglycans affects the in situ mechanical behavior of extrafibrillar matrix in bone. Mechan. Behav. Biomed. Mater. 123, 104766.

  Zhang, N., Kim, S.H., Gainullina1, A., Erlich, E.C., Onufer, E.J., Ding, J., Saunders, B.T., Dominguez, J.R., Czepielewski, R.S., Williams, J.W., Harris, C.A., Jiang, J.X.,  Segal, B., Zinselmeyer, B.S., Artyomov, M.N., Randolph, G.J., and Kim, K-W. (2021) LYVE1hi macrophages within peritoneal mesothelium promote omentum-independent ovarian tumor growth. J. Exp. Med. 218, e20210924

  Ni, Q., Hua, R., Holland, D., Tinajero, A., Han, Y., Jiang, J.X. and Wang, X. (2021) Characterization of microstructural changes on biglycan induced mice bone by low-field nuclear magnetic resonance. Applied Physics. 4, 58-67

  Quan, Y., Du, Y., Wu, C., Gu, S. and Jiang, J.X. (2021) Connexin hemichannels regulate redox potential via metabolite Exchange and protect lens against cellular oxidative damage. Redox Biol. 46, 102102.

  Quan, Y., Du, Y., Tong Y., Gu, S., and Jiang, J.X. (2021). Connexin gap Junctions and hemichannels in modulating lens redox homeostasis and oxidative stress in cataractogenesis. Antioxidants 10, 1374.

   Quan, Y., Du, Y., Wu, C., Gu, S., and Jiang, J.X. (2021) Data on connexin 43 hemichannels regulation of cellular redox in lens. Data in Brief 39, 107572.

  Li, Y., Francisca, M.A., Quan, Y., Li, Z., Gu, S. and Jiang, J.X. (2022) Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection. Star Protocol 3, 101060

   Acosta, F.M., Stojkova, K., Zhang, J., Huitron, E.I.G., Jiang, J.X., Rathbone, C.R., and Brey, E.M. (2022) Engineering functional vascularized beige adipose tissue from microvascular fragments isolated from healthy and diabetic adipose tissue.  J. Tissue Engineer. 13, 1-17. 

  Zhao, D., Riquelme, M.A., Guda, T., Tu, C., Xu, H., Gu, S., and Jiang, J.X.  (2022) Connexin hemichannels with prostaglandin release in anabolic function of bone to mechanical loading. eLife 11, e74365.  

   Emoto, T., Lu, J., Sivasubramaniyam, T., Khan, A.B., Abow, A.A., Schroer, S.A., Maan, H., Hyduk, S., Althagafi, M., McKee, T., Fu, F., Shabro, S., Ulndreaj, A., Chiu, F., Paneda, E., Pacheco, S., Wang, T., Li, A., Jiang, J.X., Libby, P., Husain, M., Wang, B., Rubin, B.B., Cybulsky, M., and Robbins, C.S. (2022) CSF-1 producing sinusoidal endothelial cells and mesenchymal stromal cells maintain monocytes within a perivascular bone marrow niche. Immunity 55, 862-870

  Li, Z., Quan, Y., Gu, S., and Jiang, J.X. (2022) Beyond the Channels: Adhesion of Aquaporin 0 and Connexin 50 in Lens Development. Front. Cell Dev. Biol. 10, 866980

  Zhao, D., Hua, R., Riquelme, M.A., Cheng, H., Guda, T., Xu, H., Gu, S., and Jiang, J.X. (2022) Osteocytes regulate bone anabolic response to mechanical loading via activation of integrin α5. Bone Res. 10, 49.

  Hua, R., Gu, S., and #Jiang, J.X. (2022) Connexin 43 hemichannels regulate osteoblast to osteocyte differentiation. Front Cell Dev. Biol. 10:892229.