Steven D. Dallas, Ph.D.
Associate Professor in Clinical Laboratory Sciences
Dr. Steven Dallas is an Associate Professor in Clinical Laboratory Sciences. He is also the Director of the Microbiology Laboratory at University Hospital.
Dr. Dallas earned his PhD in Medical Microbiology from Texas Tech University Health Sciences Center and is board certified in Medical and Public Health Microbiology by the American Board of Medical Microbiology and by the New York State Department of Health in bacteriology, virology, parasitology, mycology, and mycobacteriology. Dr. Dallas is an experienced clinical microbiology educator, researcher, and practitioner. He is the section chief (medical director) of the microbiology laboratory at University Hospital. He also serves as the CLIA laboratory director for San Antonio Metro Health Laboratory. His research interests include Group A Streptococcus, Mycoplasma pneumoniae, susceptibility testing methods, and collaborative research involving resistance mechanisms. He is actively involved in teaching pathology residents, infectious disease fellows, pharmacy residents, podiatry residents, medical students, and PA studies and medical laboratory sciences students in the UT Health School of Health Professions. Dr. Dallas has been an invited speaker at local, regional, national, and international meetings including ASM and ASCP.
His interests include:
- Antimicrobial susceptibility breakpoints
- Atmospheric pollen and mold counts
- Clinical Laboratory Management
- Clinical Laboratory Science
- Clinical Microbiology
- Detection and characterization of glycoproteins.
- Detection of proteins
- Development of ELISA techniques
- Protein and immunologlobulin detection
- Purification of bacterial toxins
- Radiolabeling and autoradiography of proteins
- Transmission electron microscopy, bacteriophage
- 1998 - PhD - Medical Microbiology - Texas Tech University Health Sciences Center
- 1981 - BS - Medical Technology - Abilene Christian University
- 9/2015 - Associate Professor and Associate Professor - UTHSCSA, Clinical Laboratory Sciences, San Antonio
- 12/2013 - Director, Microbiology Laboratory - University Hospital, San Antonio, TX, Pathology, San Antonio
- 10/1999 - Consulting Microbiologist - Dosher Memorial Hospital, Southport, NCSouthport
Instruction & Training
- 8/2014 - Present, Post Graduate Rotation Supervision, University Hospital
- 8/2014 - Present, Post Graduate Rotation Supervision, University Hospital
- 8/2014 - Present, Post Graduate Rotation Supervision, University Hospital
- 4/2014 - Present, Post Graduate Rotation Supervision, University Hospital
- 3/2011 - Present, Clinical Pathology, The University of Texas Health Science Center
- 1/2010 - Present, Laboratory Medicine, The University of Texas Health Science Center
- 10/2009 - Present, Clinical Microbiology, The University of Texas Health Science Center
- 9/2009 - Present, Adv Microbiology, The University of Texas Health Science Center
- 1/2009 - Present, Medical Microbiology, The University of Texas Health Science Center
- 1/2009 - Present, Medical Microbiology Lab, The University of Texas Health Science Center
Research & Grants
Funding Agency CDC Title HSC20170678H: Sentinel surveillance for macrolide-resistant Mycoplasma pneumoniae at select sites in the United States Status Active Period 12/2017 - 12/2019 Role Co-Investigator Grant Detail The basic design of the proposed surveillance will be for each clinical site to freeze remnant specimens from samples tested by the Biofire Respiratory Panel and ship them to UAB at specified intervals over a 12-month period where they will be tested for macrolide resistance by real-time PCR and cultured for M. pneumoniae. Isolates will be tested for phenotypic antimicrobial susceptibilities in vitro, and genotyped. Additionally, clinical patient data will be collected for each specimen in electronic format and sent to UAB. An initial draft of the data form which is currently under development is provided as a separate attachment to this email so you will be aware of what information will need to be recorded. A database will be developed and analyzed to address the research questions in the CDC priorities. Depending on the results and availability of funding, it is possible that CDC may want to continue this surveillance beyond the initial 12-month period.
Funding Agency Clinical Microbiology Institute, Wilsonville, OR Title Protocol Number: HSC20170224N Title: CMI CF-301 Lysin vs. Staphylococcus aureus Survey Collection Status Active Period 1/2017 - 3/2017 Role Principal Investigator Grant Detail CMI CF-301 Lysin vs. Staphylococcus aureus Survey Collection. I reviewed and collected MRSA and MSSA isolates from University Hospital for the study specified below. The study will pay $1250 to my T account and will later share conclusions from the multi-site study.
CMI is collecting 50 strains of Staphylococcus aureus from each of six US laboratories, three European laboratories and 2 laboratories in South America. These strains will be used to determine the susceptibility of a new lysin compound vs. 25 S. aureus oxacillin susceptible and 25 S. aureus oxacillin resistant obtained from each of the 10 sites.
Sites will collect all strains from recent clinical isolates isolated in 2016. Frozen stocks may be included as long as all cultures were collected in 2016 or early 2017.
Each site should collect 25 MRSA and 25 MSSA each isolate should be from a unique patient culture. Only one isolate per patient should be included. Please provide the date cultured and the culture source for each isolate (See Excel file for recording this information). If mecA or PBP 2a test results are available please include the test and the result on the worksheet.
Waites KB, Ratliff A, Crabb DM, Xiao L, Qin X, Selvarangan R, Tang YW, Zheng X, Dien Bard J, Hong T, Prichard M, Brooks E, Dallas SD, Duffy L, Mixon E, Fowler KB, Atkinson TP. Macrolide-Resistant Mycoplasma pneumoniae in the United States as Determined from a National Surveillance Program: American Society for Microbiology; 2019 Jun. Waites KB, Ratliff A, Crabb DM, Xiao L, Qin X, Selvarangan R, Tang YW, Zheng X, Dien Bard J, Hong T, Prichard M, Brooks E, Dallas SD, Duffy L, Mixon E, Fowler KB, Atkinson TP. Molecular Characterization of Mycoplasma pneumoniae in the United States from a National Surveillance Program: American Society for Microbiology; 2019 Jun. Grace Lee*1, James Shurko1, Steven Dallas2, Jordan Meckel1, Bryson Duhon3. The microbiome of diabetic foot infections are associated with clinical features and long-term outcomes P2126 ECCMID; 2018 Apr. (ESCMID 2018). Ruth Serrano, MD, Infectious Disease, University of Texas Health Science Center at San Antonio, San Antonio, TX; Infectious Disease, South Texas Veterans Health Care System, san antonio, TX, Michelle Matheu, MD, Division of Infectious Diseases, Department of Internal Medicine, South Texas Veterans Health Care System, San Antonio, TX, Federico Perez, MD, Louis Stokes Cleveland VA Medical Center, Cleveland, OH, Jan E. Patterson, MD, MS, FIDSA, FSHEA, University of Texas Health Sciences Center, San Antonio, TX, Steven Dallas, PhD, The University of Texas Health Science Center San Antonio, San Antonio, TX, Susan D. Rudin, BS, Case Western Reserve University, Cleveland, OH, Robert A. Bonomo, MD, Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleavland, OH, Kristi Traugott, PharmD, University Health System, San Antonio, TX and Jose Cadena, MD/ID, University of Texas Health Science Center, San Antonio, TX. Molecular and Microbiological Evaluation of Carbapenem-resistant Enterobacteriaceae (CRE) at Two Centers in Texas: IDSA; 2017 Oct. (ID Week 2017). Grace C. Lee, PharmD, PhD1,2, James Shurko, PharmD1,2, Steven Dallas, PhD1, Yufeng Wang, PhD3, Randall Olsen, MD, PhD4, Kenneth Lawson, PhD2, James Wilson, PharmD, PhD2 and Christopher R. Frei, PharmD, MSc2,5, (1)The University of Texas Health Science Center San Antonio, San Antonio, TX, (2)The University of Texas at Austin, Austin, TX, (3)The University of Texas San Antonio, San Antonio, TX, (4)The Houston Methodist Hospital and Research Institute, Houston, TX, (5)UT Medicine, San Antonio, TX. Genome-Wide Analysis of S. aureus Strains to Identify Variants Associated with Infection versus Colonization: IDSA; 2017 Oct. (ID Week 2017). James Shurko, PharmD1,2, Steven Dallas, PhD1, Bryson M. Duhon, Pharm.D.1,2, Jordan Meckel, PharmD Candidate2, Chiou-Miin Wang, PhD1, Chun-Lin Lin, PhD1, Nicholas Lucio, B.S.1, Nameer Kirma, PhD1 and Grace C. Lee, PharmD, PhD2, (1)The University of Texas Health Science Center San Antonio, San Antonio, TX, (2)The University of Texas at Austin, Austin, TX. Identification of Pathogens Directly from Diabetic Foot Infections by Shotgun Metagenomic Sequencing: IDSA; 2017 Oct. (ID Week 2017). Dallas SD
Smith LA. What Is That Bug In My Blood? San Diego, CA: American Society for Clinical Laboratory Science Annual Meeting and Clinical Lab Expo 2017; 2017 Jul. (ASCLS). Matheu M, Serrano R, Dallas SD, Kelly C, Perez, F, Cadena J,. Evaluation of Carbapenem-resistant Enterobacteriaceae at Two Academic Medical Centers in Texas Abstract #58040: Infectious Diseases Society of America; 2016 Oct. (IDSA). Dallas SD, Cadena Zuluaga JA, Patterson JE, Kelly C, Traugott K. In vitro susceptibility testing of essential oils against carbapenem-resistant Enterobacteriaceae and selected ATCC strains Abstract 56874 2016: IDSA; 2016 Oct. (ID Week 2016). Lee GC
Frei CR. Comparative Genomics and Antimicrobial Resistance Determinants of Community-associated Staphylococcus aureus Strains in South Texas Abstract #4732 Washington, DC: American Society for Microbiology; 2016 Jun. (ASM Microbe General Meeting June-16-20, Boston, 2016). Boyd N, Lee GC, Frei CR, Trevino L, Trevino S, Dallas, SD Retzloff C, Du L, Jahangir C. Risk Factors for Community Associated MRSA versus MSSA Skin and Soft Tissue Infections in Primary Care Clinics: A Case-Case-Control Study 2015-A-2667-ASM-ICAAC Podium Presentation Washington, DC: American Society for Microbiology; 2015 Sep. (ICAAC ICC 2015 Sep 17-21, 2015). Nazarullah A, Dallas SD, Jorgensen JH. A Lick that Kills: Case of a Rapidly Fatal Pasteurella multocida Sepsis; 2012 Sep. (College of American Pathologists Annual Meeting 2012). Labreche, MJ., Lee, G., Parchman, ML., Mortensen, EM., Dallas SD., Du, LC., Nyren, NR., Pena, J., Rocha, G., Esparza, S., Finnie, M. Frei, CR. Cost of Treatment Failure Among Adult Outpatients with Moderate or Complicated Community-Acquired Methicillin-Resistant Staphylococcus aureus (CA MRSA) Skin Infections: A STARNET Study; 2012 Jan. Dallas SD, McElmeel LM, Jorgensen JH, Fulcher LC. Development of Doxycycline MIC and Disk Diffusion Interpretive Breakpoints for Streptococcus pneumoniae; 2011 May. (American Society for Microbiology 108th General Meeting Abstracts ISBN 978-1618392695). Dallas SD, Rupar DG, Clegg HW, Sautter RL. Exserohilum sp. Infections in Immunocompromised Pediatric Patients; 2008 Jun. (American Society for Microbiology 108th General Meeting Abstracts ISBN 978-1555814939). Sautter R, Dallas SD, Oblack DL, LeBar WD, Carolinas Pathology Group, Charlotte, MC, Presbyterian Laboratory Services, Charlotte, NC, verteran Affairs Medical Center, Cincinnati, OH, Hospital Consolidated Laboratories, Southfield, MI. CLSI (NCCLS) Guidelines for S. aureus testing of Penicillin, Should they be modified?; 2006 May. (American Society for Microbiology 106th General Meeting Abstracts ISBN 1-55581-389-5). Dallas SD, Avery AL, Pekarek PM, Mills TJ, Neal WJ, Smallbrook AG, Hejna J, Presbyterian Laboratory services, BD Diagnostics, Sparks MD. Comparison of BD Phoenix to Biomerieux Vitek for the identification and susceptibility testing of common bacterial isolates; 2005 May. (American Society for Microbiology 105th General Meeting Abstracts ISBN 1-55581-338-0).
Dallas SD. Manual of Clinical Microbiology 12th Edition Book Chapter (Chapter 3, Microscopy). In: Dallas SD. Manual of Clinical Microbiology 12th Edition. Washington, DC: ASM Press; 2019. p. 16-27. Dallas SD. Textbook of Diagnostic Microbiology, 5th Edition, Laboratory Manual ISBN 978-0-323-08989-0. In: Dallas, Steven D. Mahon, Connie R. Lehman, Donald C. Manuselis, George. Textbook of Diagnostic Microbiology, 5th Edition. Maryland Heights, Missouri: Saunders, Elsevier; 2014.
Dallas SD (consulting editor), Tille P (author). Bailey and Scott`s Diagnostic Microbiology, 13th Edition. ISBN 9780323083300 Maryland Heights, MO: Elsevier; 2013. 1060 p.
Beckwith, D., Campos, J., Carroll, K., Cornish, N., Craft, D., Dallas S., Desjardins, M., Dunne, M., Gilchrist, M., Hall, G., Hodinka, R., Janda, M., Mann, L., McFadden, G., Miller, M., Shortridge, V., Sifuentes-Osnornio, J., Snyder, J., Stellrecht, K., Thomson, R., Valsamakis, A., Wang, D. Clinical Microbiology in the 21st Century - Keeping the Pace 2008 Jan;:1-16.
Srinivasan A, Lee GC, Torres NS, Hernandez K, Dallas SD, Lopez-Ribot J, Frei CR, Ramasubramanian AK. High-throughput microarray for antimicrobial susceptibility testing Biotechnology Reports 2017 Nov;16:44-47. Lee GC, Frei CR, Wang Y, Dallas, SD, Olsen RJ, Lawson K. Emerging Multidrug Resistance in Community-Associated Staphylococcus aureus involved in Skin and Soft Tissue Infections and Nasal Colonization Jac-2017-0014.R1 Journal of Antimicrobial Chemotherapy 2017 Jul;72(9):2461-2468. Lee GC, Boyd N, Hall RG, Dallas SD, Du LC, Trevino LB, Retzloff C, Trevino SB, Lawson KA, Wilson JP, Olsen RJ, Wang Y, Frei CR. Predictors of community-associated Staphylococcus aureus, methicillin-resistant and methicillin-susceptible Staphylococcus aureus skin and soft tissue infections in primary-care settings Epidemiology and Infection 2016 Nov;144(15):3198-3204. Lee GC, Boyd N, Du LC, Dallas SD, Trevino LB, Trevino SB, Hall RG, Retzloff CR, Lawson KA, Wilson J, Olsen RJ, Wang Y, Frei CR. A prospective observational cohort study in primary care practices to identify factors associated with treatment failure in Staphylococcus aureus skin and soft tissue infections DOI 10.1186/s12941-016-0175-8 Annals of Clinical Microbiology and Antimicrobials 2016 Nov;15(58):1-7. Dallas SD, Greebon LJ, Qiao JC, Arora K. Answer to June 2016 Photo Quiz: An 8-Year-Old Boy with Meningoencephalitis J Clin Microbiol 2016 Jun;54(6):1673-1673. Dallas SD, Greebon LJ, Qiao JC, Arora K. Photo Quiz: An 8-Year-Old Boy with Meningoencephalitis J Clin Microbiol 2016 Jun;54(6):1411-1411. Dallas SD, Qiao J, Greebon L, Arora K. Eight Year Old Boy With Meningoencephalitis-Photo Quiz JCM control number JCM00402-14 Journal of Clinical Microbiology 2016 Mar;. Duhon BM, Dallas SD, Trammell Velasquez SA, Hand E. Staphylococcus lugdunensis Bacteremia and Endocarditis Treated with Cefazolin and Rifampin American Journal Health-Systems Pharmacology 2015 Jul;72:1114-1118. Grace C Lee, S Wesley Long, James M Musser, Stephen B Beres, Randall J Olsen, Steven D Dallas, Yury O Nunez, Christopher R Frei. Comparative Whole Genome Sequencing of Community-Associated Methicillin-Resistant Staphylococcus aureus Sequence Type 8 from Primary Care Clinics in a Texas Community Pharmacotherapy 2015 Feb;35(2). Lee GC, Dallas SD, Winkler P, Du LC, Trevino L, Trevino S, Pena J, Mann M, Munoz A, Marcos Y, Rocha G, Koretsky S, Esparza S, Finnie M, Frei CR. Prospective Comparison of Clinical Outcomes of Community-Associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA) and Methicillin-Susceptible Staphylococcus aureus (CA-MSSA) Skin and Soft Tissue Infections (SSTIS): A STARNET Study Value in Health 2014 May;. Ledeboer NA, Dallas SD, Gilligan P. Point-Counterpoint: The Automated Clinical Microbiology Laboratory: Fact or Fantasy? J Clin Microbiol 2014 Mar;. Steven D. Dallas, Lesley McGee, Brandi Limbago, Jean B. Patel, M. Leticia McElmeel, Letitia C. Fulcher, David R. Lonsway, James H. Jorgensen
. Development of doxycycline MIC and disk diffusion interpretive breakpoints and revision of the tetracycline breakpoints for Streptococcus pneumoniae Journal of Clinical Microbiology 2013 Jun;51(6):1798-1802. Matthew J. Labreche, PharmD, MS, Grace C. Lee, PharmD, Russell T. Attridge, PharmD, MS, Eric M. Mortensen, MD, MS, Jim Koeller, MS, Liem C. Du, MD, Natalie R. Nyren, PA-C, Lucina B. Trevi?o, MD, Sylvia B. Trevi?o, PA-C, Joel Pe?a, MD, Michael W. Mann,
MD, Abilio Mu?oz, MD, Yolanda Marcos, MD, Guillermo Rocha, MD, Stella Koretsky, MD, Sandra Esparza, MD, Mitchell Finnie, MD, Steven D. Dallas, PhD, Christopher R. Frei,
PharmD, MS. Treatment failure and associated costs in patients with methicillin-resistant Staphylococcus aureus skin and soft tissue infections treated in primary care settings: A South Texas Ambulatory Research Network (STARNet) study Journal of the American Board of Family Medicine 2013 Jan;26(5):508-517. Dallas SD, Hartman SK. A 69-year-old male with chronic keratitis J Clin Microbiol 2012 Aug;50(8):2545 2843-2545 2843. Clegg HW, Ryan AG, Dallas SD, Kaplan EL, Johnson DR, Norton HJ, Roddey OF, Martin ES, Swetenburg RL, Koonce EW, Felkner MM, Giftos PM. Treatment of streptococcal pharyngitis with once-daily compared with twice-daily amoxicillin: a noninferiority trial Pediatr Infect Dis J 2006 Sep;25(9):761-767. Clegg HW, Dallas SD, Roddey OF, Martin ES, Swetenburg RL, Koonce EW, Felkner MB, Ryan AG. Extrapharyngeal group A Streptococcus infection: diagnostic accuracy and utility of rapid antigen testing Pediatr Infect Dis J 2003 Aug;22(8):726-731. Dallas SD, Rolfe RD. Binding of Clostridium difficile toxin A to human milk secretory component J Med Microbiol 1998 Oct;47(10):879-888. Dallas SD and Kingsbery L. Bacteriophage plaques on primary isolation media of a urine culture growing E. coli Clinical Microbiology Newsletter 1997 Apr;.
Kirkwood, J, Dallas SD, Thomson R, Jacobs M, Ledeboer NA. Automation and the Future of Microbiology Laboratories Clinical Laboratory News 2017 Mar;43(3):18-21. Dallas SD
Mathison BA. Case #415 - March 2016, Monthly Case Studies -2016, DPDx- Laboratory Identification of Parasitic Diseases of Public Health Concern DPDx- Laboratory Identification of Parasitic Diseases of Public Health Concern 2016 Mar;.
Dallas SD. Gram Stain Errors America Society for Clinical Pathology TechSample 2004 Jan;G-12(2004):61-67.